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. 2019 Apr 29;9:6622. doi: 10.1038/s41598-019-43054-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UM171 enhances survival and proliferation of CD34⁺CD43⁺ hPSC-derived HPs. (A) Apoptosis evaluation using annexin V staining in cultures of hPSC-derived HPs expanded for 5 days. Bars are mean ± SEM for 6 independent experiments. (B–D) Assessment of proliferative potential of CD34⁺ HPs expanded for 5 days with UM171 or DMSO using Ki67 staining (B), BrdU assay (C) and cell cycle analysis (D). Bars in (B–D) are mean ± SEM for 3 independent experiments. *p < 0.05. All dot plots show CD34⁺CD43⁺ gated cells.