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. 2019 Apr 29;9:6622. doi: 10.1038/s41598-019-43054-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UM171 preferentially amplifies CD34⁺CD7⁺CD45⁺ NK cell progenitors. (A) Schematic diagram of experiments used to identify stage of NK development affected by UM171. (B) The effect of UM171 addition at stages 1 and 2 of NK cell differentiation. Bars are mean ± SEM for 3 independent experiments. (C) Phenotypic features of lymphoid progenitors generated in lymphoid cultures in the presence of UM171 or DMSO. (D) Upper panel shows the effect of UM171 on the proportion of cells with lymphoid progenitor phenotype on day 7 of NK differentiation (stage 1). Lower panel shows fold change in absolute numbers of CD34⁺ progenitors in cultures with UM171, as compared to DMSO controls. Bars are mean ± SEM for 3 independent experiments. **p < 0.01, ***p < 0.001. (E) Analysis of cell proliferation using BrdU assay in NK differentiation cultures, on days 5 and 11 of differentiation. (E) FAC-Sorting of cellular subsets from CD43⁺ HPs, on day 7 of lymphoid culture (stage 1), and assessment of their NK cell potential.