Fig. 5. Effect of siRNA-mediated gene knockdown on apolipoprotein B (apoB) mRNA editing in primary hepatocytes.

(A) siRNA-mediated gene knockdown reduced the expression of APOBEC-1, ACF, and RBM47 in primary hepatocytes. (B) siRNA-mediated gene knockdown reduced the editing efficiency of apoB mRNA C⁶⁶⁶⁶ site. Cells were transfected with APOBEC-1, ACF, and RBM47 siRNA oligonucleotides, then untreated or treated with 50 µM curcumin for 48 h. sicon: siRNA negative control. APOBEC-1, apoB editing catalytic polypeptide-1; RBM47, RNA-binding-motif-protein-47; ACF, apobec-1 complementation factor. ^*p < 0.05, ^**p < 0.01.