Figure 2.

Inhibition of HIV Production by AgoshRNA Constructs

HEK293T cells were co-transfected with 250 ng of the HIV pLAI, 1 ng of Renilla luciferase plasmid (pRL) and 25 ng of the shRNA/AgoshRNA constructs. Two days after transfection, inhibition of HIV production was determined by measuring CA-p24 levels in the culture supernatant. CA-p24 values were normalized to the Renilla luciferase activities. The ratio between the CA-p24 level and the Renilla luciferase activity in the presence of 25 ng pBS control was set at 100%. Bars represent the average values from six independent transfections, and error bars show the SD. Statistical analyses (two-way ANOVA followed by Tukey’s post hoc test) were performed, and differences among groups were considered significant when the corresponding p was < 0.05 (ns: not significant, p > 0.05; **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001).