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. 2019 Apr 8;16:452–462. doi: 10.1016/j.omtn.2019.04.001

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Northern Blotting of the AgoshRNA Processing Products

(A) Processing of the AgoshRNA variants and the corresponding shRNA set was analyzed by Northern blot with a complementary probe. Five probes were designed to analyze the intracellular processing of the overlapping AgoshRNA triplet (AgoshGag4-6), AgoshPol1, AgoshPol8, AgoshPol45, and AgoshR/T5. Ethidium bromide staining of small rRNAs and tRNAs are shown as loading controls below the blot. (B) The RNA expression level was determined by quantitation of AgoshRNA products and 5S rRNA signal in (A). The AgoshRNA/5S ratio is plotted in pixel density. Similar results were observed in at least two independent transfection experiments.