Figure 7.

Flow cytometry and Western blot analysis show that SPRK1 gene silencing suppresses VSMC apoptosis in rats with IA. Panel A and B, Flow cytometry was used to detect the apoptosis rate, and showed that there was a significant decrease in cell apoptosis belonging in VSMCs belonging to the si‐SPRK1 group; Panel C and D, protein bands and levels of apoptosis‐related factors (Bcl‐2, Bax, Caspase3) in VSMCs were detected by Western blot. *P < 0.05 vs the NC group; #P < 0.05 vs the si‐SRPK1 + IGF‐1 group; $P < 0.05 vs the SRPK1 + LY294002 group. VSMCs, vascular smooth muscle cells; IA, intracranial aneurysm; NC, negative control; SRPK1, SR protein‐specific kinases; PI3 K, phosphatidylinositol‐3 kinase