Figure 8.

RT‐qPCR and Western blot analysis show that SRPK1 gene silencing promotes VSMCs proliferation by repressing the activation of the PI3 K/Akt signaling pathway in rats with IA. Panel A, The RT‐qPCR detection of mRNA expressions of SPRK1, PI3 K, and Akt, which were mostly reduced in the si‐SPRK1 group; Panel B and C, protein bands and levels of SPRK1, PI3 K, Akt, p‐Akt, and β‐actin were detected by Western blot, which were decreased mostly in the si‐SPRK1 group. *P < 0.05 vs the NC group; #P < 0.05 vs the si‐SRPK1 + IGF‐1 group; $P < 0.05 vs the SRPK1 + LY294002 group. VSMCs, vascular smooth muscle cells; IA, intracranial aneurysm; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; SRPK1, SR protein‐specific kinases; PI3 K, phosphatidylinositol‐3 kinase