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RT‐qPCR primer sequence
| Gene | Sequence (5'−3') |
|---|---|
| SRPK1 | Forward: TTCCTCAACTGTAGGTCAGTCATTC |
| Reverse: TGTTCTTGCTCTTGTTCATCTTCAC | |
| PI3 K | Forward: CATCACTTCCTCCTGCTCTAT |
| Reverse: CAGTTGTTGGCAATCTTCTTC | |
| Akt | Forward: CTCATTCCAGACCCACGAC |
| Reverse: ACAGCCCGAAGTCCGTTA | |
| β‐actin | Forward: CACTCTTCCAGCCTTCCTTCC |
| Reverse: AGGTCTTTGCGGATGTCCAC |
RT‐qPCR, reverse transcription quantitative polymerase chain reaction; SRPK1, SR protein‐specific kinases; PI3 K, phosphatidylinositol‐3 kinase.
