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. 2018 Oct 7;25(4):452–464. doi: 10.1111/cns.13062

Figure 4.

Figure 4

TH compounds possibly induce autophagy through inhibiting mTOR and activating ULK1. Representative Western blotting image of mTOR, p70S6K, p‐p70S6K (Ser371) (A) and ULK1, p‐ULK1 (Ser 757), Atg13 (C). MCF‐7 cells were treated with 1‰ DMSO, 1 μmol/L rapamycin, 10 μmol/L FK506, 1 μmol/L TH compounds for 24 h as indicated. Quantification of relative mTOR, p70S6K, p‐p70S6K (B) expression levels in (A) and ULK1, p‐ULK1 (Ser 757), Atg13 (D) expression levels in (B). All the substances were dissolved in DMSO. Scale bars: 10 μm. Data are shown as the mean ± SEM *P < 0.05, **P < 0.01, ***P < 0.001 compared to DMSO control, n = 3