Figure 2.

Alterations in protein levels by sugars parallel alterations in mRNA levels. Rice suspension cells were cultured in Suc-containing (+S) medium for 72 h, transferred to Suc-free (−S) medium for 72 h, and transferred to +S medium for an additional 72 h. Total proteins were extracted from rice suspension cells and subjected to protein gel-blot analysis. The amount of total proteins applied in each lane was 10 μg for α-Amy and 50 μg for Act or SSP2.