Figure 6.

Phosphorylatable but slowly metabolized Glc analog activates or represses certain gene expression. Rice suspension cells were cultured in Suc-containing medium for 5 d and transferred to medium containing 40 mm Glc (+G), no Glc (−G), or 2 mm Man for 2 and 12 h. Total RNA was purified and subjected to RNA gel-blot analysis. a, SU genes as probes. b, SD genes as probes. Six membrane blots were parallel prepared. The Act, HSP86 and rDNA probes, ADH2, G3PD, and SSP2 probes, αAmy3 and D1 probes, αAmy8 and B1 probes, A3 and F1 probes, and A2 probe, were respectively hybridized to each of the six blots.