Figure 7.

Pyruvate represses SD gene expression. Rice suspension cells were cultured in Suc-containing (+S) medium for 5 d and transferred to +S medium or Suc-free (−S) medium with or without 50 mm pyruvate. Total RNA was purified and subjected to RNA gel-blot analysis. a, SU genes as probes. b, SD genes as probes. Six membrane-blots were parallel prepared. The HSP86, αAmy3 and αAmy8 probes, SSP2 and A3 probes, Act and F1 probes, G3PD, B1, and E1 probes, rRNA and D1 probes, ADH2 and A2 probes were respectively hybridized to each of the six blots.