Figure 1.

SOX11 overexpression induces growth arrest and neuronal differentiation of glioma cells. (A) Compared with ZsGreen1 empty vector lentivirus infection group (as control), SOX11 overexpression for 3 days caused growth suppression of U87, U251, and A172 cells. (B) FACS illustrated that SOX11⁺ cell cycle was arrested in G0/G1 phase significantly. Blue indicates cells within G0/G1 phase, green indicates cells within S phase, and red indicates cells within G2/M phase. Statistical difference was accepted when P < 0.05 compared to control. **P < 0.01, ***P < 0.001. (C) FACS revealed that ectopic SOX11 significantly promoted cell apoptosis 3 days after virus infection. Statistical difference was accepted when P < 0.05 compared to control. **P < 0.01. (D) Immunofluorescence staining of control and SOX11 overexpression cells with MAP2, TUBB3, SOX11, and ZIC1 primary antibodies, respectively. MAP2, TUBB3, and ZIC1 co‐expressed with SOX11 (indicated in red channel). MAP2 and TUBB3 are expressed in cytoplasm, SOX11 is only expressed in the nuclei, and ZIC1 is expressed both in nuclei and cytoplasm. Data were obtained from 10 randomly selected fields from triplicate samples