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. 2019 Feb 26;8(1):LMT04. doi: 10.2217/lmt-2018-0010

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© 2019 Lin Ma

This work is licensed under a Creative Commons Attribution-NonCommercial NonDerivative 4.0 Unported License

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Figure 2. — (A) CLDN-3 expression profile from Gene Expression Omnibus database (GDS5410) in which CSCs were isolated from H460 and allowed to redifferentiate under adherent conditions in serum-containing media for up to 24 h. (B) Sorting of SP from H460 cells using BD Aria. Verapamil was used as the negative control for SP analysis. (C) Expression of claudin-3 as well as CSCs markers in H460 and H460 SP. (D) Detection of claudin-3 protein levels between parental H460 cells and H460 tumorspheres. (E) Detection of claudin-3 protein levels among nonsquamous NSCLC cell lines including A549, H157 and H292. (F) Detection of SP percentage among various non-squamous NSCLC cell lines including A549, H157 and H292. Data of SP analysis from triplicates are presented as the mean ± standard deviation; *p < 0.05;**p < 0.01;***p < 0.001.

CSC: Cancer stem-like cell; NSCLC: Non-small-cell lung carcinoma; SP: Side-population.

Figure 2. — (A) CLDN-3 expression profile from Gene Expression Omnibus database (GDS5410) in which CSCs were isolated from H460 and allowed to redifferentiate under adherent conditions in serum-containing media for up to 24 h. (B) Sorting of SP from H460 cells using BD Aria. Verapamil was used as the negative control for SP analysis. (C) Expression of claudin-3 as well as CSCs markers in H460 and H460 SP. (D) Detection of claudin-3 protein levels between parental H460 cells and H460 tumorspheres. (E) Detection of claudin-3 protein levels among nonsquamous NSCLC cell lines including A549, H157 and H292. (F) Detection of SP percentage among various non-squamous NSCLC cell lines including A549, H157 and H292. Data of SP analysis from triplicates are presented as the mean ± standard deviation; *p < 0.05;**p < 0.01;***p < 0.001.

CSC: Cancer stem-like cell; NSCLC: Non-small-cell lung carcinoma; SP: Side-population.