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. 2019 Apr 30;39(4):BSR20190150. doi: 10.1042/BSR20190150

Figure 1. miR-140-5p is down-regulated in CML patients and CML cell lines.

Figure 1

(A) qRT-PCR was used to detect and compare the miR-140-5p expression in CML patients’ PBMCs with healthy donors’ PBMCs. *P < 0.001 vs normal PBMCs. Normalized against an internal control U6 RNA. (B) FISH for detection of miR-140-5p in CML patients’ PBMCs and healthy donors’ PBMCs. Blue staining represents the nucleus and red staining indicates miR-140-5p. Scale bar  =  64 μm. Right panel shows the number of FISH-positive cells of three independent experiments (per 200 cells) *P < 0.001 vs normal PBMCs. (C) The expression levels of miR-140-5p were assessed in CML cell lines (K562 and KCL22) and healthy donors’ PBMCs. ***P < 0.001, **P < 0.01 vs normal PBMCs.