Figure 6. miR-140-5p induces CML cell apoptosis by suppressing SIX1 in CML cells.

(A,B) K562 cells were transfected with pcDNA3.1-SIX1, negative control pcDNA3.1-vector, si-SIX1, or si-NC. PKM2 mRNA and protein levels were detected using qRT-PCR and western blotting. β-actin was used as an internal control. Bottom panel shows densitometric analysis of three independent experiments. ^**P < 0.01, ^***P < 0.001 vs corresponding control. (C) ChIP-qPCR was used to detect binding of SIX1 to the PKM2 promoter region in K562 cells. Arrowheads indicate the position of primers used for ChIP-PCR. ^**P<0.01 vs IgG. (D) K562 cells were co-transfected with PKM2 promoter reporter construct and either pcDNA3.1-SIX1 or pcDNA3.1-NC. Luciferase reporter assays were performed. ^*P < 0.01 vs pcDNA3.1-vector.