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. 2019 Apr 5;17(6):4693–4702. doi: 10.3892/etm.2019.7470

Figure 5.

Figure 5.

RUNX2 overexpression reduces the suppressive effects of miR-153 on SK-BR-3 and BT-549 cells. SK-BR-3 and BT-549 cells were co-transfected with miR-153 mimic, and pcDNA3.1-RUNX2 plasmid or blank pcDNA3.1 vector, respectively. The (A) mRNA and (B) protein expression levels of RUNX2 were detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The effect on (C) SK-BR-3 and (D) BT-549 cell growth was analyzed by cell counting kit-8 assay. The effect on (E) SK-BR-3 and (F) BT-549 cell migration was analyzed using the wound healing assay (magnification, ×40). (G) The effect on SK-BR-3 and BT-549 cell invasion was analyzed using the transwell invasion assay (magnification, ×200). The protein expression levels of E-cadherin, N-cadherin and vimentin in (H) SK-BR-3 and (I) BT-549 cells were determined by western blotting. **P<0.01 vs. miR-153 + blank. SK-BR-3 and BT-549, human breast cancer cell lines; miR, microRNA; miR-153 + blank, breast cancer cell lines SK-BR-3 and BT-549 co-transfected with miR-153 mimic and blank pc-DNA3.1 vector; miR-153+RUNX2, breast cancer cell lines SK-BR-3 and BT-549 co-transfected with miR-153 mimic and pc-DNA3.1-RUNX2 plasmid.