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. 2019 Apr 18;41(6):3292–3304. doi: 10.3892/or.2019.7131

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Expression of USP9X is downregulated in prostate cancer tissues. (A) Strong cytoplasmic USP9X staining in a sample of normal prostate tissue. (B) Strong cytoplasmic and nuclear USP9X staining in a sample of normal prostate tissue. (C) USP9X downregulation (negative staining in most cells) in a sample of prostate cancer tissue. (D) Positive USP9X staining in a case of prostate cancer tissue; magnification, ×400. (E) IHC scores for the USP9X staining in the immunohistochemistry images of Fig. 1A-D. (F) Western blot analysis and (G) the relative quantification of USP9X in 8 paired prostate cancer/normal tissues. *P<0.05 vs. adjacent tumor tissue, using paired Student's t-test. The data are presented as the mean ± standard deviation. USP9X, ubiquitin-specific protease 9X; IHC, immunohistochemistry.