Figure 5.

Comparison of AaCA1 and AaCA2 with other α-CAs. Residues located within the active site of CAs (Eriksson and Liljas, 1992; Hewett-Emmett and Tashian, 1996) are indicated with asterisks. Residues conserved among at least four of the six sequences are capitalized. Sequences used are from Anabaena (Soltes et al., 1997), mouse (Curtis et al., 1983), Chlamydomonas (abbreviated as Chlamy in the figure; Fukuzawa et al., 1990b), and Chlorella (Satoh et al., 1998). Sequences were aligned using the pileup program of the GCG suite of sequence analysis programs. The large proportion of hydrophobic amino acids within the first 20 amino acids of AaCA1 and AaCA2 (boxed regions) suggests the presence of an endoplasmic reticulum signal sequence (von Heijne, 1983; Fukuzawa et al., 1990b). Periods represent gaps introduced within sequences to maximize alignments.