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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Nat Metab. 2019 Mar 11;1:404–415.

Figure 2. CRISPR-based genetic screen identifies no cytosolic NADPH producers beyond oxPPP, ME1 and IDH1.

Figure 2.

(a) Schematic of screen, which is based on comparing gene essentiality both in WT versus ΔG6PD knockout cells and in ΔG6PD versus ΔG6PD/ΔIDH1 knockout cells. (b) Top 10 conditional essential genes in ΔG6PD compared to WT HCT 116 cells based on Z score. (c) Top 10 conditional essential genes in ΔG6PD/ΔIDH1 compared to ΔG6PD cells based on Z score. (d) Cell growth of targeted pooled CRISPR knockout of DCXR, ENOX1 or ME1 in the ΔG6PD/ΔIDH1 background (mean ± SD, n = 4). After puromycin selection of transfected cells, only ME1, but not the other tested screen hits, was validated in the targeted knockout experiments.