Figure 4. Fatty acid synthesis is maintained in HCT116 G6PD knockout cells.

(a) Fraction of different non-essential fatty acid species synthesized de novo based on extent of labeling from U-¹³C-glucose (for full labeling patterns, see Supplementary Fig. 3, n = 2). (b) NADPH consumption for fatty acid synthesis (n = 3). (c,d) Palmitoleic acid (C16:1) and citrate labeling fraction in cells fed 100 μM [U-¹³C]palmitate (conjugated to BSA) for 24 h (n = 6). (e) NADPH production by the oxPPP (measured using ¹⁴C-CO₂ release from [1-¹⁴C] and [6-¹⁴C]glucose, n = 4) and the upper limit of production by ME1 (measured based on [U-¹³C-] lactate production from [U-¹³C]glutamine, n = 3) and NADPH consumption flux for fatty acid synthesis (n = 3). Data are mean ± SD with n indicating the number of biological replicates.