Figure 7.

Uptake of Glc by sycamore cells during anoxia and normoxia. Eight grams of reference cells were incubated into flasks containing 200 mL of a fresh Suc-free culture medium supplied with 2 mm Glc and bubbled with N₂. In one flask N₂ was replaced by O₂ after 4 h of anoxia (arrow). The evolution of Glc uptake by cells was determined via the measurement of the Glc present in the culture medium, according to the method of Bergmeyer et al. (1974). The values expressed as concentrations of Glc present in the incubation medium are from a representative experiment chosen in a series of five.