Fig. 2.

Effects of Papaver nudicaule extract NW90 on the expression of PGE2, COX2, and NOS2. RAW264.7 cells were treated with NW90 extract (250 and 500 μg/ml) and LPS (100 ng/ml) for 24 h. a The PGE2 production was measured in the culture supernatants. b Cells were lysed and the expression of COX2 and NOS2 was examined by western blotting. β-actin was used as an internal control. c, d Quantification of COX2 and NOS2 expression with normalization to the β-actin protein levels were analyzed by densitometry analysis of every band using the ImageJ software. Data are presented as mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistically significant differences compared with the group treated with LPS alone