Fig. 3.

Overview of differentially expressed genes (DEGs) between control and stress-treated samples. a Volcano plots depict DEGs for each treatment-by-time combination. Up-regulated DEGs are indicated by blue dots, down-regulated DEGs are indicated by red dots. Total number of DEGs are shown in the upper left and right corner for significant up- and down-regulated DEGs. DEGs that do not exceed the threshold of |log₂FC| >1 and FDR ≤1% are depicted in grey. b Overlap of DEGs at 6 h that are also differentially expressed at 24 h for each treatment in percent. Bars show overlaps of all DEGs, up-regulated and down-regulated DEGs separately. c Venn diagram showing the overlap between DEGs responsive to water deficit, salt and combined stress after 6 h of treatment. Arrows indicate number and direction of DEGs. d Venn diagram showing the overlap between DEGs after 24 h of treatment. Arrows indicate number of up- and down-regulated DEGs