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. 2019 Apr 30;12:103. doi: 10.1186/s13068-019-1445-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — In vitro binding experiments using recombinant protein rPoxMBF1 and the promoter regions of target genes. Each EMSA reaction system contained 0–2.0 μg of Trx–His–S-tagged rPoxMBF1 and ~ 40 ng of each candidate probe. The same amounts of purified Trx–His–S fusion protein, BSA, and ITS sequence were, respectively, employed as negative controls