Fig. 7.

The 3C protease and variants were expressed in DEF cells. a DEF cells were transfected with pEGFP-3C, pEGFP-3C-H38A, pEGFP-3C-C144A, and pEGFP-N1 for 24 h. DEF cells were then stained with DAPI for DNA (blue) and 3C antibody (red) as indicated. b DEF cells were transfected with pcDNA3.1-myc-His (−) A-3C and pcDNA3.1-myc-His (−) A for 24 h. Then DEF cells were stained with DAPI for DNA (blue) and 3C antibody (green) as indicated