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. 2019 Apr 29;19:405. doi: 10.1186/s12885-019-5609-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miRNA-140-5p could target TGFBRI and IGF1R. Validation of TGFBRI and IGF1R as targets of miRNA-140-5p. The sequence of potential binding site of miRNA-140-5p in the 3’UTR of TGFBRI and IGF1R mRNA (a) and (b). Validation of the miRNA-140-5p binding target using luciferase reporter assays. HEK-293 T cells were transfected with an miRNA-140-5p mimic or a control oligonucleotide (NC) and a dual luciferase reporter containing a wild-type promoter or a mutant promoter of TGFBRI and IGF1R. The luciferase activities in the cells with the mutant promoter/NC were significantly higher than those with the miRNA-140-5p mimic-wild-type reporter (a and b). TGFBRI and IGF1R in G401 cell lysis was pulled down and enriched with a miRNA-140-5p-specific probe and then detected using RT-PCR, the amount of IGF1R and TGFBRI were higher in the miR-140-5p probe group than control group(c)