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. 2019 Apr 23;12:99. doi: 10.3389/fnmol.2019.00099

Figure 6.

Figure 6

A8 cells presumably receive GABAergic input from tyrosine hydroxylase (TH)-positive cells in the OFF IPL. (A) Maximum projection of an Ier5-GFP whole-mount retina, labeled for TH (cyan). The inset shows the magnified view of the area marked in (A). Arrows point to perisomatic TH-positive rings, surrounding putative AII cells (Debertin et al., 2015) but not A8 cells (magenta). (B,C) Same staining as in (A) in a single scan of a vertical slice. Please note the prominent co-fasciculation of TH- and GFP-positive dendrites of dopaminergic and A8 amacrine cells, respectively. (D–G) Maximum projection of GFP-labeled A8 cells (D), TH-stained ier5-GFP retina (E), GABAA-R α3 staining (F) and their overlay (G). The upper inset shows the magnified view. Arrows point to the colocalization (G) of the three channels (D–F). (H) Intensity profile of the colocalized area, indicating GABAergic input from dopaminergic amacrine cells to the glycinergic A8 cell. The upper inset shows the single scan of the magnified area shown in the inset of (G). Scale bar: (A), 20 μm; inset: 10 μm; (B–G), 10 μm; inset, 2.5 μm.