. Author manuscript; available in PMC: 2019 Apr 30.

Published in final edited form as: Cell Calcium. 2018 Dec 18;78:35–47. doi: 10.1016/j.ceca.2018.12.008

Table 1.

Serum free media components for the different protocols.

		Pure culture medium + BDM (500 ml)	Mixed culture medium with salts (480 ml)	Mixed culture medium with salts + BDM (480 ml)	Mixed culture medium with salts + BLB (480 ml)

Medium	M199	485ml	95.2ml	95.2ml	95.2ml
	BSA	50mg
	ITS	5ml
	PS	10ml	4.8ml	4.8ml	4.8ml
	Creatine	–	524 mg (20% for final)	524 mg (20% for final)	524 mg (20% for final)
	L-carnitine	–	316 mg (20% for final)	316 mg (20% for final)	316 mg (20% for final)
	Taurine	–	500.8 mg (20% for final)	500.8 mg (20% for final)	500.8 mg (20% for final)
	BDM	505 (252.5)mg	–	–	–
Salts (78% of the total	Water	–	1000	1000	1000
substance)	NaCl	–	6.78g	6.43g	6.78g
	KCl	–	400mg	400mg	400mg
	MgCl₂	–	800μl	800μl	800μl
	NaH₂PO₄H₂₀	–	124.2mg	124.2mg	124.2mg
	Glucose	–	1.008g	1.008g	1.008g
	HEPES	–	4.76g	4.76g	4.76g
	CaCl₂	–	1.8ml	1.8ml	1.8ml
	NaHCO₃	–	2.184g	2.184g	2.184g
	BDM	–	–	1.314g	–
	Blebbistatin	–	–	–	7.308 mg