Table 2.
Global and local Ca2+ characteristics.
| Parameter | Fresh | BDM | Blebbistatin | Mixed culture medium with salts |
|---|---|---|---|---|
| Time to peak [ms] | 138 ± 15 | 138 ± 35 | 122 ± 12 | 122 ± 13 |
| t50 [ms] | 251 ± 22 | 284 ± 61 | 205 ± 9 | 291 ± 43 |
| t90 [ms] | 362 ± 22 | 603 ± 89* | 321 ± 10 | 721 ± 26* |
| Cycle length [ms] | 402 ± 24 | 809 ± 142* | 411 ± 14 | 1542 ± 43* |
| 50% duration [ms] | 25 ± 0.89 | 28 ± 1 | 24 ± 0.8 | 28 ± 1 |
| Amplitude difference | 1.3 ± 0.03 | 1.295 ± 0.03 | 1.13 ± 0.01 | 1.2 ± 0.01 |
| Calcium release length [μm] | 4.42 ± 0.16 | 2 ± 0.12* | 3.4 ± 0.08 | 1.84 ± 0.1* |
| Time in [ms] between calcium release and former peak | 280 ± 9 | 362 ± 43* | 269 ± 14 | 938 ± 32* |
| Ca2+ releases/μm/sec | 0.14 ± 0.02 | 0.123 ± 0.03 | 0.17 ± 0.04 | 0.1 ± 0.03 |
| Number of cells | 12 | 6 | 8 | 12 |
| Number of calcium releases | 107 | 47 | 61 | 60 |
| Number of rabbits | 6 | 3 | 3 | 3 |
*
p < 0.05 vs. fresh cells.