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. Author manuscript; available in PMC: 2019 Apr 30.
Published in final edited form as: J Bone Miner Res. 2018 Nov 5;34(2):375–386. doi: 10.1002/jbmr.3594

Fig. 4.

Fig. 4.

The p.Arg160* variant disrupts the FAM92A/Chibby1 complex. (A) COS-7 cells were cotransfected with nonfluorescent MYO10NANOTRAP construct, GFP-Chibby1 (bait, green), and/or tdTomato-FAM92A or tdTomato-FAM92AR160* (Preys, red) constructs. Single channels are also shown as inverted grayscale images. Accumulations at the tip of bait and prey are shown with an arrowhead. Stars indicate the absence of accumulation of prey at the filopodia tip. Scale bar=10 μm. (B) Quantification of nanoSPD 2.0 assay. CIB2 was used as a negative control. ****p ≤ 0.0001; ns=nonsignificant; AU=Arbitrary Unit. (C) Co-immunoprecipitation of FAM92A variants with Chibby1 and FAM92A variants. The red arrows indicate the expected sizes of fusion proteins. *IgG heavy chain.