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. Author manuscript; available in PMC: 2019 Apr 30.
Published in final edited form as: J Bone Miner Res. 2018 Nov 5;34(2):375–386. doi: 10.1002/jbmr.3594

Fig. 5.

Fig. 5.

The p.Arg160* variant impairs the recruitment and the colocalization of FAM92A with Chibby1 at the base of cilia. HeLa cells were cotransfected with GFP-Chibby1 (green) and tdTomato-FAM92A or tdTomato-FAM92AR160* (red) constructs. Ciliogenesis was induced by 24-hour serum starvation. HeLa cells were immunostained with acetylated α-tubulin antibody (blue), a marker of cilia, and counterstained with DAPI (grayscale). Single channel and merge images are shown. Accumulations of Chibby1 and Fam92a variants are indicated with white and red arrows. Scale bars=2 μm.