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Purification of the TOM complex from Arabidopsis. Total outer mitochondrial membrane protein from Arabidopsis was solubilized by 5% (w/v) digitonin and was directly analyzed by two-dimensional BN-PAGE/Tricine SDS-PAGE as described under “Materials and Methods.” The gel was silver stained. Sizes of standard proteins are given on the right in kilodaltons. A scheme of the gel is presented in the bottom part of the figure. TOM subunits are marked in black.
