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. 2019 Jan 29;8:117. [Version 1] doi: 10.12688/f1000research.16802.1

Figure 6. Spinal cord cultures grown in 96-well microplates are amenable to modulators of myelination.

Figure 6.

A. Representative immunofluorescence images of 96-well microplate myelinating cultures (28 DIV) stained for MBP, phosphorylated neurofilament (SMI31) and nuclei (DAPI). Cells were untreated or treated with Activin-A or FGF9 as described. B. Percentage change in myelination, axon density and nuclei count after treatment with Activin-A or FGF9. Bars represent average values from 5 independent cultures, each with 5 wells of technical replicates (wells), +/- s.e.m.. Raw data attached. Cells were allocated to different treatments as described in Materials and Methods. * p < 0.05, **p < 0.001.