FIGURE 5.

ICW characterization of SV40 polyomavirus. SVG-A cells were infected with SV40 at indicated MOIs for 72 h. Cells were then fixed and stained for VP1 expression. SV40 VP1 expression was analyzed by ICW and FFU quantitation as in Figure 1. (A) ICW plates were stained with CellTag 700 (red) and a LI-COR-800 secondary antibody to target VP1 (green). (B) For each experimental assay, a minimum of triplicate samples were analyzed and the percent infection or percent response for each treatment was normalized to the DMSO control, which was set to 100%. For each experimental assay, the percent infection or percent response of a given MOI was normalized to the highest MOI, which was set to 100%. Error bars are representative of the standard deviation from the mean. (C) ICW and FFU data were compared across identical conditions by linear regression and an experimental correlation was determined using a Pearson correlation coefficient. The gray shaded region represents the confidence interval (standard error of the linear regression). Error bars represent the standard deviation from the mean. Data are representative of three independent experiments.