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. 2019 Apr 17;13:1187–1196. doi: 10.2147/DDDT.S196172

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© 2019 Wang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Inhibition of autophagy abolished the protective effect of TET against BUP in SH-SY5Y cell. SH-SY5Y cells were exposed to 200 μM TET with 500 μM BUP and BUP+TET+3MA. (A) Cell viability was determined using CCK-8 assay in SH-SY5Y cells in 72 hrs. (B) Apoptotic cells were detected with Annexin V and PI double staining. (C) The apoptosis cell rates were calculated. (D) Relative fluorescence expression levels were quantified by Ki67 and DAPI staining. (E) The number of Ki67 positive cells was counted. **P<0.01 compared with DMSO group; ^##P<0.01 compared with 500 μM BUP group; ^{^^}P<0.01 compared with BUP+TET group.

Abbreviations: 3MA, 3-methyladenine; BUP, bupivacaine; monodansylcadaverine; TET, tetramethylpyrazine.