FIGURE 1.

Inhibitory effects of blocking primers at varying concentrations against shrimp sequence amplification (A), eukaryotic microorganisms (B), zooplankton (C) templates. Samples were amplified with six different blocking primers against three shrimps at concentrations of 0.4, 0.8, and 1.6 μM. Experiments were repeated thrice. Primers (lanes) with preferred inhibition efficacy are marked with red rectangles. Please find the detailed methods of sample collection and PCR protocol from Sections “Sample Collection and DNA Extraction” and “Amplification With Blocking Primers” in the main text.