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. 2019 Apr 23;10:830. doi: 10.3389/fmicb.2019.00830

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Copyright © 2019 Liu, Qi, Jiang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The taxonomic composition of eukaryotic microorganisms in shrimp (A,C) and oyster (B,D) amplified with the blocking primer at various concentrations. C and D, details of class composition of “non-host” in shrimp and oyster samples (the relative abundance of ASVs >0.01%) when the primer concentration is 0.6 μM. Numbers above each bar chart are reads counts, and were all grouped in class level. “+0,” “+0.4,” and “+0.6 μM” implying that the samples were amplified with the blocking primer, X-BP2-DPO, at different concentrations. “X1-3” and “L1-3” are six different shrimp and oyster samples, respectively.