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. 2019 Apr 23;10:830. doi: 10.3389/fmicb.2019.00830

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Copyright © 2019 Liu, Qi, Jiang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in the percentage of taxa in shrimp samples. (A) Changes in the percentage of host reads in shrimp samples amplified with the blocking primer, X-BP2-DPO at various concentrations. (B–E) Changes in percentage of the four dominant eukaryotes reads [Cyclotella (B), Cryptomonadales (C), uncultured Halteria (D), and Oligotrichia (E)] in shrimp samples amplified with the blocking primer, X-BP2-DPO at various concentrations. “+0,” “+0.4,” and “+0.6 μM” imply that the samples were amplified with the blocking primer, X-BP2-DPO at the concentrations of 0, 0.4, and 0.6 μM in the PCRs. The data was analyzed by applying Tukey’s test and Levene’s test (p = 0.013).