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. 2019 Apr 17;13:1221–1231. doi: 10.2147/DDDT.S201818

Figure 4.

Figure 4

Aloin (ALO) inhibited the activation of the Akt-mTOR-P70S6K signalling pathway induced by rhHMGB1. HGC-27 cells were treated with different doses of rhHMGB1 for 12 h (A), or with 2 µg/ml of rhHMGB1 at different times (3, 6, 12, 24 h) (B), the phosphorylatio and total amount of Akt, mTOR, P70S6K, S6, 4EBP1 were detected by Western blotting. The relative levels were normalized by β-actin. Data are presented as mean ± SD of three independent experiments. *p<0.05 and ** p<0.01 vs control group. HGC-27 cells were pre-treated with different doses of ALO for 2 h, and then stimulated with 2 µg/ml of rhHMGB1 for 12 h (C). The phosphorylation and total amount of Akt, mTOR, P70S6K, S6, 4EBP1 were detected by Western blotting. The relative levels were normalized by β-actin. Data are presented as mean ± SD of three independent experiments. ap<0.05 and bp<0.01 vs control group, cp<0.05 and dp<0.01 vs rhHMGB1 group.