Table 1.
Primers and PCR conditions used to amplify and sequence the vast majority of genomic sequence including the reported SNPs on VKORC1
| Forward primer | Reverse primer | Annealing temp (°C) |
Annealing time (min) | Product size (bp) |
|---|---|---|---|---|
| F1-GCCAGCAGGAGAGGGAAATA | R1-AGTTTGGACTACAGGTGCCT | 58 | 60 | 290 |
| F2-GCAGGCACCTGTAGTCCAA | R2-CGCTTACCCTATGCCAAGTC | 58 | 60 | 842 |
| F3-TGATCCGCTGGTCTCTAGGT | R3-GAGAGCACTAAGCCCGTCAG | 58 | 60 | 786 |
| F5-GTCTGGCTGTGCGTGATG | R5-GCCATAGCGCCCGATTAATT | 58 | 45 | 313 |
| F6-AGGCGTTAGCATAATGACGG | R6-GGGTGGAACCAGGTTAGGAC | 60 | 60 | 725 |
| F7-GTCCTAACCTGGTTCCACCC | R7-CCCTCCAAGGGACTGGTCT | 60 | 60 | 995 |
| F9-GAATACGTGCGTAAGCCACC | R9-GGTTCAGACTTGGCTGATTG | 60 | 45 | 659 |