Figure 1.
The relationship between size exclusion chromatography and nuclear magnetic resonance (NMR) spectroscopy. Plasma was separated by size exclusion chromatography. Choline-containing phospholipid was quantified in each fraction using a kit from Wako. Eluted fractions were also quantified by NMR spectroscopy at LabCorp (formerly LipoScience) using an optimized version (LP4) of NMR LipoProfile. The average size particles from each of the gel filtration fraction quantified by NMR is shown in the table to the right and are consistent with those obtained by NMR. LDL: low-density lipoprotein; VLDL: very low-density lipoprotein; HDL: high-density lipoprotein; FPLC: fast protein liquid chromatography