Figure 2.

Ectopic expression of NRF3 suppresses breast cancer cell proliferation and migration. (A) Western blot was performed to determine the overexpression of NRF3 in SKBR3 and MDA-MB-231 cells by transfected ectopic NRF3-expressed plasmids. β-actin was used as loading control. (B) Colony form assay for SKBR3 and MDA-MB-231 cells transfected with NRF3-overexpressed plasmids or empty vector as 800 cells per well. The colonies were stained with crystal violet solution. (C) MTT assay was performed to evaluate the proliferation variation between MDA-MB-231 cells transfected with empty vector (blue) and NRF3-expressed plasmid (red). Error bar indicated SD (**P<0.01). (D) Cell migration assay was performed to detect the migration ability of SKBR3 and MDA-MB-231 cells with NRF3-overexpressed plasmids or empty vector by transwell. The cells were stained with crystal violet solution (left and middle panels). The quantification of the cell number migrated per view at 24 hours after transfected (right panel). pcDNA3.1 was used as negative control. Error bar indicated SD (**P<0.01). Three independent experiments were performed for all assays.

Abbreviations: NRF3, Nuclear Factor, Erythroid 2 Like 3.