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. 2019 Apr 11;11:3111–3123. doi: 10.2147/CMAR.S200566

Figure 5.

Figure 5

Rescue experiments were performed to confirm that HMGB1 is the functional target of miR-665 in RB cells. Y79 cells were co-transfected with miR-665 mimics and either pc-HMGB1 or pcDNA3.1. WERI-RB-1 cells were co-transfected with miR-665 inhibitor and either si-HMGB1 or si-NC. After different incubation times, the cells were collected and used in the following experiments. (A) At 72 h post transfection, HMGB1 protein was measured by Western blot analysis. *P<0.05 compared with miR-NC. **P<0.05 compared with miR-665 mimics + pcDNA3.1. #P<0.05 compared with NC inhibitor. ##P<0.05 compared with miR-665 inhibitor + si-NC. (B-D) Cell proliferation, colony formation, and apoptosis were examined by using the CCK-8, clonogenic, and flow cytometric analysis, respectively. On day 15, a photo of colonies formed was taken. *P<0.05 compared with miR-NC. **P<0.05 compared with miR-665 mimics + pcDNA3.1. #P<0.05 compared with NC inhibitor. ##P<0.05 compared with miR-665 inhibitor + si-NC.