Figure 1.

Expression and association miR-216b and FOXM1 in NSCLC tissues and cells. (A) qRT-PCR was performed to detect the expressions of miR-216b and FOXM1 mRNA in 30 cases of NSCLC tissues and four kinds of cell lines (A549, H460, H1299, and normal human bronchial epithelial cell line HBE). (B) Western blot was used to detect FOXM1 protein level in NSCLC tissues and cell lines. Relative grayscale values of Western blotting were calculated to analyze the protein levels. U6 and GAPDH were used as internal controls, respectively. (C) Spearman’s correlation analysis was carried out to investigate the relationship between miR-216b and FOXM1 mRNA in 30 cases of NSCLC tissues. (D) Spearman’s correlation analysis was carried out to investigate the relationship between miR-216b and FOXM1 protein in 30 cases of NSCLC tissues. (E) Kaplan–Meier analysis of overall survival rates of patients with NSCLC according to miR-216b and FOXM1 expression status. Asterisks indicated statistically significant differences. Data were represented as means±SD. * P<0.01 vs control.

Abbreviations: FOXM1, forkhead box M1; NSCLC, non-small cell lung cancer