Figure 1.

Establishing an inducible expression system for αSyn overexpression in SK‐N‐MC cells. A, Scheme of GeneSwitch System for αSyn inducible overexpression. Incubation of GeneSwitch‐driven cells with mifepristone (MFP) can induce αSyn overexpression. B, Intracellular levels of αSyn were analyzed by Western blotting. At 24 h postinduction by MFP, there was a slight increase of αSyn levels in a dose‐dependent manner, and this overexpression was further upregulated at 48 h. The highest enhancement of αSyn overexpression is about 6‐fold higher than that of the control at 10 μmol/L of MFP. All Western blotting data were performed in three independent experiments, and the densitometrical values are presented as mean ± SEM