Table 4.

Ig kappa PCR primers.

PCR	Primer	Sequence (5′–3′)	V gene segment
IGK PCR primers
Kappa Chain Forward primers	5′GP_VK#1	GACATCCAGATGACCCAGTCTCCAT	Vk1-142
	5′GP_VK#2	GACATTGTTATGACCCAGTCTACAG	Vk4-36
	5′GP_VK#3	GCATCCAGTTGACACAGCCTCCATC	Vk1-178
	5′GP_VK#4	GTCATCCAGATGATGCAGTATTCAT	Vk3-108
	5′GP_VK#5	GATATCCAGTTGACACAGCCTGCAT	Vk1-147, Vk1-58
	5′GP_VK#6	GACATTTTGATGACCCAGTCTCCAG	Vk4-37
	5′GP_VK#7	GAAATTCAGATGACACAAACTTCCT	Vk1-99
	5′GP_VK#8	GAAGTTGTGCTGACCCAGACTCCAC	Vk2-124
	5′GP_VK#9	GACATCCAGATGATCCAGTCACCAG	Vk1-133
	5′GP_VK#10	GACATCCAGATTACTCAGACTCCAT	Vk1-174
	5′GP_VK#11	GACATCCAGATGACTCAGACTCCAT	Vk1-280, Vk1-226, Vk1-55, Vk1-51, Vk1-47, Vk1-40, Vk1-15
	5′GP_VK#12	GACATCCAGATGACTCAGACTCCGT	Vk1-272
	5′GP_VK#13	GAAACCCTGCTGACTGAGACTCCAG	Vk3-25
	5′GP_VK#14	GACATCCAGTTGACGCAGCCTCCAT	Vk1-201
	5′GP_VK#15	GATGTTCTGATGACCCAGACCCCAC	Vk2-15
	5′GP_VK#16	GATGTAGTGATGACCCAGACTCCAC	Vk2-16
	5′GP_VK#17	GATACTCAGATGACTCAGTCTCCAT	Vk1-247
	5′GP_VK#18	GAAACCCTTCTGACACAGACCCCAG	Vk3-155
	5′GP_VK#19	GATGTTGTGGTGACCCAGACCCCAC	Vk2-51, Vk2-11
	5′GP_VK#20	GATATCCAGATGACTCAGGCTCCTT	Vk1-230
	5′GP_VK#21	GACATCCAGATGATTCAGACTCCAT	Vk1-21
	5′GP_VK#22	GACATTGTGATGACTCAGTCTCCAG	Vk4-28
	5′GP_VK#23	GACATCCAGTTAACACAGCCTCCAT	Vk1-211, Vk1-159
	5′GP_VK#24	GACATCCAGTTGACCCAGTCTCCAT	Vk1-246
	5′GP_VK#25	GACATTAGGATGACCCAGACCCCAC	Vk2-85
	5′GP_VK#26	GATGTTGTATTGACCCAAACCCCAC	Vk2-14
	5′GP_VK#27	GACATCCAGATGACCCAGTCACCAT	Vk1-190
	5′GP_VK#28	GACATTGTGATGACCCAGTCTCCAG	Vk4-70, Vk4-95, Vk4-45, Vk4-41, Vk4-39, Vk4-32, Vk4-29, Vk4-90, Vk4-83, Vk4-81
	5′GP_VK#29	GAAACCCTGTTGACCCAGACTCCAG	Vk3-170
	5′GP_VK#30	GATATCCAGTTGACACAGCCTCCAT	Vk1-152
	5′GP_VK#31	GACATTGTGATGACCCAGTCACCAG	Vk4-74
	5′GP_VK#32	GAAATTGTGATGACCCAGTCTCCAG	Vk4-77
	5′GP_VK#33	GACATCCCGATGACTCAGATTCCAT	Vk1-36
	5′GP_VK#34	GACATTCAGATGACCCAGTCTCCAT	Vk1-140, Vk1-65, Vk1-254
	5′GP_VK#35	GACATACAGATGACCCAGTGTCCAT	Vk1-234
	5′GP_VK#36	GACAATGTGGTGATCCAGTCTCCAG	Vk4-69
	5′GP_VK#37	GACATCCAGTTGACACAGCCTCCTT	Vk1-214
	5′GP_VK#38	GACATCCAGATGACCCAGTCTCAAT	Vk1-144
	5′GP_VK#39	GACACCCAGATGACCCAGTCTCCAT	Vk1-145
	5′GP_VK#40	GACATCCAGATGACTCAGACTGCAT	Vk1-59
	5′GP_VK#41	GACTTCCAGATGACCCAGTCACCAT	Vk1-219
	5′GP_VK#42	GACATCCAGTTGACACAGCCTCCAT	Vk1-242, Vk1-239
	5′GP_VK#43	GACATAGTGATGACCCAGACCCCAC	Vk2-10
	5′GP_VK#44	GATGTTGTGATGACCCAGACCGCAC	Vk2-7
	5′GP_VK#45	GACATCCGGATGACTCAGACTCCAT	Vk1-179
	5′GP_VK#46	GAAAAATTACTGACTAAGACTCCAG	Vk3-263
	5′GP_VK#47	GATATCCAGATGACTCAGGCTCCCT	Vk1-267
	5′GP_VK#48	GACATCCAATTGACACAGCCTGCAT	Vk1-279, Vk1-266, Vk1-29, Vk1-18, Vk1-1
	5′GP_VK#49	GAAACCCAGCTGACTCAGACTCCAG	Vk3-281, Vk3-60, Vk3-42
	5′GP_VK#50	GATATTGTGATGACACAGACCCCAC	Vk2-20
	5′GP_VK#51	GACATCCAGTTGACCCAGACTCCAG	Vk1-126
	5′GP_VK#52	GACATCCTATTAACCCAGCCTCCCT	Vk1-101
	5′GP_VK#53	CAAATTGTGCTCACCCAGACTCCAG	Vk5-57
	5′GP_VK#54	GATGTTTTGATGACCCAGACCCCAC	Vk2-22
	5′GP_VK#55	GAAATTGTGCTTACCCAGTCTCCAG	Vk5-105
	5′GP_VK#56	GATATCCAGTTGACCCAGTCTTCCT	Vk1-117
	5′GP_VK#57	GACATCAAATTGACTCAGCCAGCAT	Vk1-233
	5′GP_VK#58	GTGTAGGAAAAAACATCACTATTAC	Vk1-245
	5′GP_VK#59	GACATCCAGATGACTCAGACTCTCT	Vk1-153
	5′GP_VK#60	GACATCCAGTTGACCCAGTCTCCCT	Vk1-131, Vk1-115, Vk1-65
	5′GP_VK#61	GACCTTGTTATGACACAGTCTCCAG	Vk4-76
	5′GP_VK#62	GACATTCAGATGAGCCAGTCTCCAT	Vk1-184
	5′GP_VK#63	GACATCCAGTTGATGCAGCCTCCAT	Vk1-253
	5′GP_VK#64	GATGTTGTGATGACCCAGACCCCAC	Vk2-3
	5′GP_VK#65	GACATCCAACTGACACAACCTGCAT	Vk1-261, Vk1-203
	5′GP_VK#66	GACATCCAGATGACTCAGTCTCCCT	Vk1-130, Vk1-128
	5′GP_VK#67	GACATCCAAATGACTCAGGTTCCAT	Vk1-166
	5′GP_VK#68	GATGTTTTGTTGTCCCAGACCCCAC	Vk2-2
	5′GP_VK#69	GACATCCAGTTGACACAGCCTGCAT	Vk1-275, Vk1-271, Vk1-225, Vk1-54, Vk1-50, Vk1-46, Vk1-45, Vk1-39, Vk1-35, Vk1-24, Vk1-20, Vk1-14, Vk1-8
	5′GP_VK#70	GACATCCAGCTGACACAGCTTGCAT	Vk1-11
	5′GP_VK#71	GCGTTGCCCTGACACAGTCCCCAGC	Vk6-164
Reverse primers	3′ Ck 156_OUT_GP	GTGTTGTCCTTGCTGTCCTGATC	Ck (1st PCR)
	3′Ck_internal_GP	GTTCAGAGCCATCCACCTTCCAC	Ck (2nd PCR)