Table 6.
Ig lambda cloning primers.
| Cloning PCR | Primer | Sequence (5′–3′) | V gene segment |
|---|---|---|---|
| IGL PCR primers | |||
| Lamda Chain Forward primers | SL_5′GP_VL#1 | tttctagtagcaactgcaaccggttctctctcgCAGCTTGTGCTGACTCAGTCACCCT | VL4-92 |
| SL_5′GP_VL#2 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTGCTGACACAGCCGTCTT | VL3-39 | |
| SL_5′GP_VL#3 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTGCTCAAACAGCCACCTT | VL3-30 | |
| SL_5′GP_VL#4 | tttctagtagcaactgcaaccggttctctctcgCAGCCGGTGCTCACTCAACCACCCT | VL3-134 | |
| SL_5′GP_VL#5 | tttctagtagcaactgcaaccggttctctctcgCAGCCTGTGCTGACTCAGCTTCCCT | VL8-87 | |
| SL_5′GP_VL#6 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTACTGACACAGCCATCTT | VL3-27 | |
| SL_5′GP_VL#7 | tttctagtagcaactgcaaccggttctctctcgCAGGAACTGGTGACTCAGGAACCCT | VL5-74 | |
| SL_5′GP_VL#8 | tttctagtagcaactgcaaccggttctctctcgTCTTACACCCTCACTCAACCTCCCT | VL3-55, VL3-31, VL3-47, VL3-41, VL3-9 | |
| SL_5′GP_VL#9 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTATTCACACAGCCACCTT | VL3-6 | |
| SL_5′GP_VL#10 | tttctagtagcaactgcaaccggttctctctcgCAGCCTGTGCTGAAACAGTCACCCT | VL4-138, VL4-84, VL4-82 | |
| SL_5′GP_VL#11 | tttctagtagcaactgcaaccggttctctctcgCAGGTTGTGCTGACTCAGTCACCCT | VL4-80 | |
| SL_5′GP_VL#12 | tttctagtagcaactgcaaccggttctctctcgCAGACTTCAGTCACCCAGGAGCCAT | VL7-129 | |
| SL_5′GP_VL#13 | tttctagtagcaactgcaaccggttctctctcgCAGCCTGTGCTGACTCAGCTGCCCT | VL8-104, VL8-85 | |
| SL_5′GP_VL#14 | tttctagtagcaactgcaaccggttctctctcgCAGGCAGTGCTGAGTCAGCCGCCCT | VL1-125 | |
| SL_5′GP_VL#15 | tttctagtagcaactgcaaccggttctctctcgCAGGATCTGGTGACTCAGGAACCCT | VL5-79, VL5-78 | |
| SL_5′GP_VL#16 | tttctagtagcaactgcaaccggttctctctcgCTGGCTGTGGTAACTCAGGAATCTT | VL6-102 | |
| SL_5′GP_VL#17 | tttctagtagcaactgcaaccggttctctctcgTCGTATGTGCTGACGCAGCCATCTT | VL3-21 | |
| SL_5′GP_VL#18 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTGCTGACACAGCCATCTT | VL3-4 | |
| SL_5′GP_VL#19 | tttctagtagcaactgcaaccggttctctctcgCAGTCTGGCCTAAGTCAGGAAGCTT | VL1-96 | |
| SL_5′GP_VL#20 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTACTCACACAGCCACCCT | VL3-53 | |
| SL_5′GP_VL#21 | tttctagtagcaactgcaaccggttctctctcgAAGGCTGTGGTGACTCAGGAATCTT | VL6-115 | |
| SL_5′GP_VL#22 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTACTCACACAGTCACCTT | VL3-24 | |
| SL_5′GP_VL#23 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTGCTGACGCAGCCATCTT | VL3-50, VL3-34, VL3-32, VL3-16, VL3-48, VL3-45, VL3-42, VL3-13, VL3-10 | |
| SL_5′GP_VL#24 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTACTCACACAGCCACCTT | VL3-52, VL3-29 | |
| SL_5′GP_VL#25 | tttctagtagcaactgcaaccggttctctctcgCAGGCTGTGGTGACTCAGGAACCTT | VL6-71 | |
| SL_5′GP_VL#26 | tttctagtagcaactgcaaccggttctctctcgCAGCTTGTGCTGACTCAGTCACCTT | VL4-121 | |
| SL_5′GP_VL#27 | tttctagtagcaactgcaaccggttctctctcgCAGAATGTAGCGACCCAGGTATCCT | VL5-106 | |
| SL_5′GP_VL#28 | tttctagtagcaactgcaaccggttctctctcgCAGACTGTGGTGACCCAGGTATTCT | VL5-122 | |
| SL_5′GP_VL#29 | tttctagtagcaactgcaaccggttctctctcgCAGGCAGTGCTGACTCAGCTGCCCT | VL1-95 | |
| SL_5′GP_VL#30 | tttctagtagcaactgcaaccggttctctctcgCAGGCAGTGCTGACTCAGCCGCCCT | VL1-109 | |
| SL_5′GP_VL#31 | tttctagtagcaactgcaaccggttctctctcgTCTTATATCTTGACACAGCCACCCT | VL3-19 | |
| SL_5′GP_VL#32 | tttctagtagcaactgcaaccggttctctctcgTCTTACATCTTGACACAGCCTCCCT | VL3-43_VL3-35 | |
| SL_5′GP_VL#33 | tttctagtagcaactgcaaccggttctctctcgCAGGCTGTGGTGACTCAGGAATCTT | VL6-132, VL6-98, VL6-69, VL6-68, VL6-116 | |
| SL_5′GP_VL#34 | tttctagtagcaactgcaaccggttctctctcgCAGGATCTGGTAACTCAGGAACCTT | VL5-99 | |
| SL_5′GP_VL#35 | tttctagtagcaactgcaaccggttctctctcgTCCTATGTGCTCACACAGCCACCTT | VL3-8 | |
| SL_5′GP_VL#36 | tttctagtagcaactgcaaccggttctctctcgCAGCCTGTAGTGACTCAACCACCCT | VL5-139 | |
| Reverse primers | 3_GP_XhoI_JL_1_4_7 | ggcttgaagctcctcactcgagggcgggaacagagtgaccaagggggaagccttgggctgaccg AGGACGGTCAGCTTGGTG | JL1, JL4, JL7 |
| 3_GP_XhoI_JL_2_5_9 | ggcttgaagctcctcactcgagggcgggaacagagtgaccaagggggaagccttgggctgaccg AGGACCGTCAGCCTGGTT | JL2, JL5, JL6 | |
| 3_GP_XhoI_JL3_6_10 | ggcttgaagctcctcactcgagggcgggaacagagtgaccaagggggaagccttgggctgaccg AGGACGGTCAGCTTGGTT | JL3, JL6, JL10 | |
| 3_GP_XhoI_JL8 | ggcttgaagctcctcactcgagggcgggaacagagtgaccaagggggaagccttgggctgaccg AGGACTGTCAGGTCGGTT | JL8 | |
| 3_GP_XhoI_JL11 | ggcttgaagctcctcactcgagggcgggaacagagtgaccaagggggaagccttgggctgaccg AGGACGGTCACCTTGGTC | JL11 | |