Table 8.
Genetic and binding specificity analysis of guinea pig mAb variable region sequences‡.
| VDJ segments_CDRH3 | VJ segments_CDRL3 | Binding specificity | |||||||
|---|---|---|---|---|---|---|---|---|---|
| mAb | Lineage # | length (AA) | VH Mut (%) | CDRH3 AA seq | length (AA) | VL Mut (%) | CDRL3 AA seq | (ELISA/BLI) | |
| nAbs | CP2 | 1 | VH3-197_D6_JH4_CDR3_17 | 7.5 | TRNLLLSGVESTGAFDV | VL3-41_JL_3_CDR3_13 | 2.0 | AITLGSGSIFQSV | Env (V3) |
| CP94 | 1 | VH3-197_D6_JH4_CDR3_17 | 8.4 | TRNLLLSGVESTGAFDV | VL3-41_JL_3_CDR3_13 | 2.0 | AITLGSGSIFQSV | Env (V3) | |
| CP6 | 2 | VH3-197_D18_JH4_CDR3_12 | 11.0 | AKNKGETASFDV | VL3-41_JL_1_CDR3_13 | 6.0 | SIARVSGNNIQWV | Env (V3) | |
| CP10 | 3 | VH3-197_D3_JH4_CDR3_15 | 9.1 | AKNGDTSNGETTPDV | VL3-41_JL_1_CDR3_13 | 6.0 | TVAHVSGNAFQWV | Env (V3) | |
| CP67 | 4 | VH3-197_D17_JH4_CDR3_17 | 7.3 | AKNLLLSEATSTGAFDV | VL4-82_JL_1_CDR3_9 | 12.0 | AVGHSAGWV | Env (V3) | |
| Non- nAbs | CP3 | 5 | VH3-183_D15_JH4_CDR3_18 | 8.0 | TRGPYYRWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 6.6 | AVGYNTGWV | Env, unknown |
| CP7 | 5 | VH3-183_D15_JH4_CDR3_18 | 11.2 | ARAPYYKWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 8.4 | AVGHSAGWV | Env, unknown | |
| CP13 | 5 | VH3-183_D15_JH4_CDR3_18 | 6.9 | ARGAYYRWGTWSLYYFDV | VL4-82_JL_1_CDR3_9 | 5.1 | AVGYSAGWV | Env, unknown | |
| CP37 | 5 | VH3-183_D15_JH4_CDR3_18 | 7.7 | ARGAYYKWGTWTLYYFDL | VL4-82_JL_1_CDR3_9 | 7.6 | AVGHSAGWV | Env (unknown) | |
| CP53 | 5 | VH3-183_D15_JH4_CDR3_18 | 11.4 | ARAPYYKWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 7.6 | AVGHSAGWV | Env (unknown) | |
| CP61 | 5 | VH3-183_D15_JH4_CDR3_18 | 7.7 | ARGPYYRWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 5.8 | AVGYSAGWV | ∗Env (unknown) | |
| CP62 | 5 | VH3-183_D15_JH4_CDR3_18 | 11.5 | ARAPYYKWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 9.8 | AVGHSAGWV | ∗Env (unknown) | |
| CP73 | 5 | VH3-183_D15_JH4_CDR3_18 | 11.1 | ARGPYYRWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 7.6 | AVGYSAGWV | Env (unknown) | |
| CP91 | 5 | VH3-183_D15_JH4_CDR3_18 | 7.6 | ARGPYYKWGTWSLYYFDI | VL4-82_JL_1_CDR3_9 | 7.3 | AVGYSAGWV | ∗Env (unknown) | |
| CP92 | 5 | VH3-183_D15_JH4_CDR3_18 | 7.0 | ARGAYYKWGTWTLYYFDL | VL4-82_JL_1_CDR3_9 | 8.0 | AVGYSAGWI | Env (unknown) | |
| CP68 | 6 | VH3-183_D15_JH2_CDR3_18 | 8.3 | QEGSYYKWGMGMTSNHHA | VL4-82_JL_1_CDR3_9 | 6.6 | AVGYSAGWV | Env (unknown) | |
| CP58 | 7 | VH3-230_D31_JH2_CDR3_7 | 5.1 | TTTGLTY | Vk1-201_Jk2_CDR3_9 | 3.6 | WQYDKLPLT | Env (unknown) | |
| CP63 | 7 | VH3-230_D17_JH2_CDR3_7 | 2.9 | TTTGLGY | Vk1-201_Jk2_CDR3_9 | 3.5 | WQYDKLPLT | Env (unknown) | |
| CP66 | 8 | VH3-139_D27_JH4_CDR3_7 | 2.9 | ARMVVDV | Vk4-83_Jk2_CDR3_9 | 0.7 | MQDYNPPYT | Env (unknown) | |
| CP82 | 9 | VH3-80_D31_JH4_CDR3_17 | 3.7 | ARDGWEEYMWGGSFLDL | Vk4-28_Jk3_CDR3_11 | 1.9 | LQYYDFPNT | Env (unknown) | |
‡Heavy- and light-chain sequences of mAbs cloned were analyzed by IgBLAST and assigned to the closest V, D, and J germline genes. Clonal lineage criteria are shown in Figure 2. Somatic hypermutation level (Mut %) were calculated as percentage of nucleotide sequence divergence from germline V gene sequences. ∗ELISA/BLI negative. Specificity inferred by clonal lineage analysis, e.g., belonging to the same lineage with BG505 SOSIP binding clones.