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. 2019 Apr 15;11:3253–3264. doi: 10.2147/CMAR.S193793

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© 2019 Wang et al.

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CDKN3 increased ESCA resistance to cisplatin in vitro and in vivo. (A) Comparison of CDKN3 mRNA between sensitive and resistant tumors. (B–C) EC109R (B) and OE19R (C) cells displayed greater resistance to cisplatin than their parental cells. (D) Comparison of CDKN3 mRNA between resistant cells and their parental cells. (E) CDKN3 protein expression was inhibited in cisplatin-resistant ESCA by shCDKN3 as determined by western blots. (F–G) Decreased CDKN3 expression weakened ESCA chemoresistance in EC109R (F) and OE19R (G) as determined by CCK8 assay. (H–I) CDKN3 Knockdown enhanced cisplatin-induced ESCA apoptosis (H) and the quantitative results (I). (J–K) CDKN3 inhibition regulated cisplatin-induced xenograft tumor volumes (J) and the quantitative results (K). **P<0.01, ***P<0.001.

Abbreviations: EC109R, cisplatin-resistant EC109 cell line; OE19R, cisplatin-resistant OE19 cell line; NC, negative control; shCDKN3, cells transfected with shCDKN3 plasmid; IC50, half maximal inhibitory concentration.